Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: activity in the absence of added hydrogen peroxide.
Identifieur interne : 000E21 ( Main/Exploration ); précédent : 000E20; suivant : 000E22Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: activity in the absence of added hydrogen peroxide.
Auteurs : I C Kuan [États-Unis] ; M. TienSource :
- Archives of biochemistry and biophysics [ 0003-9861 ] ; 1993.
Descripteurs français
- KwdFr :
- Champignons (enzymologie), Chélateurs (pharmacologie), Consommation d'oxygène (MeSH), Formiates (métabolisme), Glyoxal (métabolisme), Glyoxylates (métabolisme), Modèles biologiques (MeSH), Oxalates (métabolisme), Oxydoréduction (MeSH), Oxygène (métabolisme), Peroxidases (effets des médicaments et des substances chimiques), Peroxidases (métabolisme), Peroxyde d'hydrogène (métabolisme), Phénolsulfonephtaléine (MeSH), Piégeurs de radicaux libres (MeSH), Spectroscopie de résonance de spin électronique (MeSH), Superoxide dismutase (métabolisme), Superoxydes (métabolisme).
- MESH :
- effets des médicaments et des substances chimiques : Peroxidases.
- enzymologie : Champignons.
- métabolisme : Formiates, Glyoxal, Glyoxylates, Oxalates, Oxygène, Peroxidases, Peroxyde d'hydrogène, Superoxide dismutase, Superoxydes.
- pharmacologie : Chélateurs.
- Consommation d'oxygène, Modèles biologiques, Oxydoréduction, Phénolsulfonephtaléine, Piégeurs de radicaux libres, Spectroscopie de résonance de spin électronique.
English descriptors
- KwdEn :
- Chelating Agents (pharmacology), Electron Spin Resonance Spectroscopy (MeSH), Formates (metabolism), Free Radical Scavengers (MeSH), Fungi (enzymology), Glyoxal (metabolism), Glyoxylates (metabolism), Hydrogen Peroxide (metabolism), Models, Biological (MeSH), Oxalates (metabolism), Oxidation-Reduction (MeSH), Oxygen (metabolism), Oxygen Consumption (MeSH), Peroxidases (drug effects), Peroxidases (metabolism), Phenolsulfonphthalein (MeSH), Superoxide Dismutase (metabolism), Superoxides (metabolism).
- MESH :
- chemical , drug effects : Peroxidases.
- chemical , metabolism : Formates, Glyoxal, Glyoxylates, Hydrogen Peroxide, Oxalates, Oxygen, Peroxidases, Superoxide Dismutase, Superoxides.
- chemical , pharmacology : Chelating Agents.
- enzymology : Fungi.
- Electron Spin Resonance Spectroscopy, Free Radical Scavengers, Models, Biological, Oxidation-Reduction, Oxygen Consumption, Phenolsulfonphthalein.
Abstract
Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. We show here that glyoxylate is capable of supporting Mn peroxidase activity without added H2O2. This glyoxylate-supported activity is dependent upon Mn2+ and dioxygen. The involvement of superoxide is demonstrated by inhibition by superoxide scavenging agents, superoxide dismutase, or tetranitromethane. The addition of catalase resulted in dioxygen evolution, indicating that H2O2 is an intermediate in the reaction. Formate is one of the oxidation products of glyoxylate as detected by the formate dehydrogenase assay. The generation of H2O2 in the presence of Mn2+ and Mn peroxidase results in Mn3+ formation. Consequently, we show that the direct reaction between glyoxylate and Mn3+ also results in formate formation. The stoichiometry of this reaction approaches 1:1. Electron spin resonance, spin-trapping studies show formation of the formate radical CO2-. in the reaction of Mn3+ and glyoxylate.
DOI: 10.1006/abbi.1993.1238
PubMed: 8387747
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Tien</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8387747</idno><idno type="pmid">8387747</idno><idno type="doi">10.1006/abbi.1993.1238</idno><idno type="wicri:Area/Main/Corpus">000E10</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E10</idno><idno type="wicri:Area/Main/Curation">000E10</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E10</idno><idno type="wicri:Area/Main/Exploration">000E10</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: activity in the absence of added hydrogen peroxide.</title><author><name sortKey="Kuan, I C" sort="Kuan, I C" uniqKey="Kuan I" first="I C" last="Kuan">I C Kuan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.</nlm:affiliation><orgName type="university">Université d'État de Pennsylvanie</orgName><country>États-Unis</country><placeName><settlement type="city">University Park (Pennsylvanie)</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Tien, M" sort="Tien, M" uniqKey="Tien M" first="M" last="Tien">M. Tien</name></author></analytic><series><title level="j">Archives of biochemistry and biophysics</title><idno type="ISSN">0003-9861</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chelating Agents (pharmacology)</term><term>Electron Spin Resonance Spectroscopy (MeSH)</term><term>Formates (metabolism)</term><term>Free Radical Scavengers (MeSH)</term><term>Fungi (enzymology)</term><term>Glyoxal (metabolism)</term><term>Glyoxylates (metabolism)</term><term>Hydrogen Peroxide (metabolism)</term><term>Models, Biological (MeSH)</term><term>Oxalates (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxygen (metabolism)</term><term>Oxygen Consumption (MeSH)</term><term>Peroxidases (drug effects)</term><term>Peroxidases (metabolism)</term><term>Phenolsulfonphthalein (MeSH)</term><term>Superoxide Dismutase (metabolism)</term><term>Superoxides (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Champignons (enzymologie)</term><term>Chélateurs (pharmacologie)</term><term>Consommation d'oxygène (MeSH)</term><term>Formiates (métabolisme)</term><term>Glyoxal (métabolisme)</term><term>Glyoxylates (métabolisme)</term><term>Modèles biologiques (MeSH)</term><term>Oxalates (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Oxygène (métabolisme)</term><term>Peroxidases (effets des médicaments et des substances chimiques)</term><term>Peroxidases (métabolisme)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Phénolsulfonephtaléine (MeSH)</term><term>Piégeurs de radicaux libres (MeSH)</term><term>Spectroscopie de résonance de spin électronique (MeSH)</term><term>Superoxide dismutase (métabolisme)</term><term>Superoxydes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Formates</term><term>Glyoxal</term><term>Glyoxylates</term><term>Hydrogen Peroxide</term><term>Oxalates</term><term>Oxygen</term><term>Peroxidases</term><term>Superoxide Dismutase</term><term>Superoxides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chelating Agents</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Formiates</term><term>Glyoxal</term><term>Glyoxylates</term><term>Oxalates</term><term>Oxygène</term><term>Peroxidases</term><term>Peroxyde d'hydrogène</term><term>Superoxide dismutase</term><term>Superoxydes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chélateurs</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electron Spin Resonance Spectroscopy</term><term>Free Radical Scavengers</term><term>Models, Biological</term><term>Oxidation-Reduction</term><term>Oxygen Consumption</term><term>Phenolsulfonphthalein</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Consommation d'oxygène</term><term>Modèles biologiques</term><term>Oxydoréduction</term><term>Phénolsulfonephtaléine</term><term>Piégeurs de radicaux libres</term><term>Spectroscopie de résonance de spin électronique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. We show here that glyoxylate is capable of supporting Mn peroxidase activity without added H2O2. This glyoxylate-supported activity is dependent upon Mn2+ and dioxygen. The involvement of superoxide is demonstrated by inhibition by superoxide scavenging agents, superoxide dismutase, or tetranitromethane. The addition of catalase resulted in dioxygen evolution, indicating that H2O2 is an intermediate in the reaction. Formate is one of the oxidation products of glyoxylate as detected by the formate dehydrogenase assay. The generation of H2O2 in the presence of Mn2+ and Mn peroxidase results in Mn3+ formation. Consequently, we show that the direct reaction between glyoxylate and Mn3+ also results in formate formation. The stoichiometry of this reaction approaches 1:1. Electron spin resonance, spin-trapping studies show formation of the formate radical CO2-. in the reaction of Mn3+ and glyoxylate.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8387747</PMID><DateCompleted><Year>1993</Year><Month>06</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-9861</ISSN><JournalIssue CitedMedium="Print"><Volume>302</Volume><Issue>2</Issue><PubDate><Year>1993</Year><Month>May</Month></PubDate></JournalIssue><Title>Archives of biochemistry and biophysics</Title><ISOAbbreviation>Arch Biochem Biophys</ISOAbbreviation></Journal><ArticleTitle>Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: activity in the absence of added hydrogen peroxide.</ArticleTitle><Pagination><MedlinePgn>447-54</MedlinePgn></Pagination><Abstract><AbstractText>Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. We show here that glyoxylate is capable of supporting Mn peroxidase activity without added H2O2. This glyoxylate-supported activity is dependent upon Mn2+ and dioxygen. The involvement of superoxide is demonstrated by inhibition by superoxide scavenging agents, superoxide dismutase, or tetranitromethane. The addition of catalase resulted in dioxygen evolution, indicating that H2O2 is an intermediate in the reaction. Formate is one of the oxidation products of glyoxylate as detected by the formate dehydrogenase assay. The generation of H2O2 in the presence of Mn2+ and Mn peroxidase results in Mn3+ formation. Consequently, we show that the direct reaction between glyoxylate and Mn3+ also results in formate formation. The stoichiometry of this reaction approaches 1:1. Electron spin resonance, spin-trapping studies show formation of the formate radical CO2-. in the reaction of Mn3+ and glyoxylate.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kuan</LastName><ForeName>I C</ForeName><Initials>IC</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tien</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1-P42ES04922-01</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Arch Biochem Biophys</MedlineTA><NlmUniqueID>0372430</NlmUniqueID><ISSNLinking>0003-9861</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002614">Chelating Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005561">Formates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016166">Free Radical Scavengers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006038">Glyoxylates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010070">Oxalates</NameOfSubstance></Chemical><Chemical><RegistryNumber>11062-77-4</RegistryNumber><NameOfSubstance UI="D013481">Superoxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>50NP6JJ975</RegistryNumber><NameOfSubstance UI="D006037">Glyoxal</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical><Chemical><RegistryNumber>I6G9Y0J1OJ</RegistryNumber><NameOfSubstance UI="D010637">Phenolsulfonphthalein</NameOfSubstance></Chemical><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002614" MajorTopicYN="N">Chelating Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004578" MajorTopicYN="N">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005561" MajorTopicYN="N">Formates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016166" MajorTopicYN="N">Free Radical Scavengers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006037" MajorTopicYN="N">Glyoxal</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006038" MajorTopicYN="N">Glyoxylates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010070" MajorTopicYN="N">Oxalates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010100" MajorTopicYN="N">Oxygen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010101" MajorTopicYN="N">Oxygen Consumption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010637" MajorTopicYN="N">Phenolsulfonphthalein</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013481" MajorTopicYN="N">Superoxides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>5</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8387747</ArticleId><ArticleId IdType="pii">S0003-9861(83)71238-5</ArticleId><ArticleId IdType="doi">10.1006/abbi.1993.1238</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><noCountry><name sortKey="Tien, M" sort="Tien, M" uniqKey="Tien M" first="M" last="Tien">M. Tien</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Kuan, I C" sort="Kuan, I C" uniqKey="Kuan I" first="I C" last="Kuan">I C Kuan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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